Ways to improve empty, full and damaged capsids separation

Presenter: Aleš Štrancar

Date: October 27, 2020

Gene Therapy for Rare Disorders Europe 2020 (digital event)

Bullet points:

• Buffer optimisation and proper column ligand selection (CIM QA, CIM PrimaS or new CIM EF) allows for >90% full capsid production
• Introduction of Specimen allows for production columns checking before use  
• Using ultracentrifuge with set of HPLC detectors allows for prompt orthogonal verification of the full capsid purity