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The Nuclear Pore Complex pp 51–66Cite as

Nuclear Pore Complex Assembly Using Xenopus Egg Extract

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2502))

Abstract

Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and how to analyze the process using Western blot or immunofluorescence based assays. The function of proteins can be conveniently assayed by high-efficient antibody mediated depletion.

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Author information

Authors and Affiliations

  1. Institute of Biochemistry and Molecular Cell Biology, Medical School, RWTH Aachen University, Aachen, Germany

    Guillaume Holzer & Wolfram Antonin

Authors
  1. Guillaume Holzer
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  2. Wolfram Antonin
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Corresponding author

Correspondence to Wolfram Antonin .

Editor information

Editors and Affiliations

  1. Department of Biosciences, Durham University, Durham, UK

    Martin W. Goldberg

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Holzer, G., Antonin, W. (2022). Nuclear Pore Complex Assembly Using Xenopus Egg Extract. In: Goldberg, M.W. (eds) The Nuclear Pore Complex. Methods in Molecular Biology, vol 2502. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2337-4_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2337-4_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2336-7

  • Online ISBN: 978-1-0716-2337-4

  • eBook Packages: Springer Protocols

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